Webinar on June 2, 2021, 3:00 pm UTC+2
High-speed 3D Phototransient Imaging over Large Volumes-of-View
Photothermal microscopy often struggles with imaging large fields-of-view or dynamically evolving systems. I will discuss our ongoing work to address this challenge via an all-optical methodology that implements a lock-in amplifier-like widefield camera which allows high frequency photothermal signal modulation with conventional, slow, CMOS cameras. We combine concepts from ultrafast optics, holography and photothermal microscopy to perform 3D volumetric phototransient single particle tracking of many freely diffusing gold nanoparticles in large (100 x 100 x 100 µm) volumes-of-view at 100 frames-per-second. I will give a detailed discussion of the experimental concepts and challenges, present proof-of-concept work and discuss our ongoing experimental efforts.