Soft Matter Physics Division - Biophysics at the University of Leipzig University of Leipzig
InternalLab Safety RegulationsCell Culture Rules

Safety Levels

Safety Level 1: genetically modified organisms that do not pose a risk for human health and the environment
Examples: SVT2 cells, genetically modified cells from Karla or Casey, GFP-labeled cells

Safety Level 2: genetically modified organisms that pose a moderate risk for human health and the environment
Examples: cells from human patients that have not been tested for HIV and Hepatitis B/C

Not classified: unmodified fibroblasts (NIH3T3, BALB3T3), unmodified neurons (PC12, NG108), fish keratocytes (should be regarded as S1 to be on the safe side)

Same regulations apply for genetically altering cells and doing experiments with already modified cells! Same rules apply for cell culture lab as well as BONG, AFM and Stretcher labs!

Rules of Conduct for S1

  • Doors have to be closed
  • Appropriate protective clothing (lab coat, gloves, evtl. goggles)
  • No mouth-pipetting -> use pipette aid
  • Beware of aerosols. If you can't avoid them work under a chemical flow hood
  • Use needles and syringes only if absolutely necessary. Do not recap. Dispose in appropriate containers.
  • No drinking, eating, smoking, application of make-up, or storage of food and drinks.
  • Labs have to be clean and tidy. Only necessary items and materials at workplace.
  • Work area has to be cleaned immediately after completion of work. Work surfaces have to be disinfected with "Descosept"
  • Before and after work hands have to be washed/disinfected (see separate skin protection plan).

Transport, Storage, Disposal

  • Transport, storage, and disposal are "gentechnische Arbeiten"
  • Transport of S1 materials only in tightly sealable, unbreakable containers
  • Storage of S1 materials only in -80C freezer or in lN2 in clearly labeled vials (room 310). The vials have to be listed in the folder.
  • Any liquid or solid trash that had contact with S1 material has to be autoclaved before discarding (room 116, 20 min, 121C). After that disposal in normal trash.
  • There are special rules for the disposal of chemical waste.

Emergency Situations

  • In case of S1 material spill:
    • secure the area
    • use paper towels to soak up the spill
    • autoclave paper towels
    • disinfect with "Descosept"
  • In case of small injuries first aid kits in all labs
  • In case of skin or eye contact use emergency showers and eye rinse (room 310)
  • In case of serious injuries: Notfallzentrum/Emergency ward Phone: 17801
  • Notify Josef or Undine or BBS (Prof. Pustowoit) immediately, especially in the case of injuries
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