| Defined
migration assays to study tumor cell migration
Ralf Kemkemer1, Claudio
G Rolli1, Jun Nakanishi2
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1
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Max Planck Institute for Intelligent
Systems, Biomaterials, Heisenbergstraße 3, 70569 Stuttgart, Germany |
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2
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NIMS, MANA, Japan |
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| Contact:
| Website |
Cell migration plays a mayor role in many physiological and pathophysiological
processes such as cancer progression. We use simple devises to study single
cell migration or migration of cell collectives in spatially defined environments.
Investigation of pancreas tumor cell migration in micro-channels revealed
a drastic dependence on the structural properties of the cells' keratin
cytoskeleton. In addition, the channel geometry seems to affect the migration
phenotype if compared to the migration on micro-contact printed adhesive
lines. Collective cell migration of well-defined cell clusters with different
sizes and geometries is investigated by using bio-passivated photo-switchable
surfaces. Experiments demonstrate how cluster size and initial geometry
affects the formation of leader cells. Donut-shaped cell cluster show a
remarkable asymmetric wound healing phenotype. |
