The PhD project is focused on development of analytical protocols and bioinformatics tools for identification and quantification of Lipid Peroxidation Products (LPPs) in various in vitro and in vivo samples. Analytical developments includes application of thin layer (TLC) and liquid chromatography (LC) coupled to high resolution mass spectrometry (MS). Several software tools for specific identification of LPPs were developed to transform the overall data processing procedure from manual identification to high throughput profiling.
12/2012-10/2017 | PhD study | University of Leipzig, Leipzig, Germany
Thesis title "Development of mass spectrometry-based methods and bioinformatics solutions for high- throughput identification and relative quantification of lipid peroxidation products”
Grade 1.0 (summa cum laude)
10/2010 – 11/2012 | Master of science | University of Leipzig, Leipzig, Germany
Thesis title "Identification of lipid peroxidation products from different phospholipid classes"
10/2009 – 3/2010 | Exchange student | University of Jena, Jena, Germany
9/2006 – 6/2010 | Bachelor of science | Wuhan University, Wuhan, China
Thesis title “Investigation of freeze dried bacterial nanocellulose as drug delivery system for the model protein albumin”
1st Prize for the Best Young Investigator Contribution Talk on Lipidomics Forum 2017, 11, 2017, Borstel, Germany
Poster prize on DGMS (German Society for Mass Spectrometry) 46th annual conference, 03, 2013, Berlin, Germany
Zhixu Ni, Georgia Angelidou, Ralf Hoffmann, and Maria Fedorova. . "LPPtiger software for lipidome-specific prediction and identification of oxidized phospholipids from LC-MS datasets." Scientific Reports 7, Article number: 15138.
Zhixu Ni, Georgia Angelidou, Mike Lange, Ralf Hoffmann, and Maria Fedorova. "LipidHunter Identifies Phospholipids by High-Throughput Processing of LC-MS and Shotgun Lipidomics Datasets." Analytical Chemistry 89 (17), pp 8800–8807.
Zhixu Ni, Ivana Milic, and Maria Fedorova. "Identification of carbonylated lipids from different phospholipid classes by shotgun and LC-MS lipidomics." Analytical and Bioanalytical Chemistry 407, no. 17 (2015): 5161-5173.
Dieter Weber, Zhixu Ni, Daniel Vetter, Ralf Hoffmann, and Maria Fedorova. "Electrochemical oxidation of cholesterol: An easy way to generate numerous oxysterols in short reaction times." European Journal of Lipid Science and Technology (2015).
Sarit Anavi, Zhixu Ni, Oren Tirosh, and Maria Fedorova. "Steatosis-induced proteins adducts with lipid peroxidation products and nuclear electrophilic stress in hepatocytes." Redox Biology 4 (2015): 158-168.
Venukumar Vemula, Zhixu Ni, and Maria Fedorova. "Fluorescence labeling of carbonylated lipids and proteins in cells using coumarin-hydrazide." Redox biology 5 (2015): 195-204.
Astrid Müller, Zhixu Ni, Nadine Hessler, FalkoWesarg, Frank A. Müller, Dana Kralisch, and Dagmar Fischer. "The biopolymer bacterial nanocellulose as drug delivery system: investigation of drug loading and release using the model protein albumin." Journal of Pharmaceutical Sciences 102, no. 2 (2013): 579-592.
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