Mike Lange

Research project:
 My main work is orientated on the identification, structural elucidation and quantification of a variety of Lipid classes as well as their oxidized counterparts. Due to the high chemical and biophysical complexity of the different lipid classes plus their increasing complexity of their oxidation products (A) analysis by Liquid Chromatography coupled to Tandem Mass Spectrometry is challenging. (B) The role of these lipid species in the development and progression of metabolic diseases related to obesity, such as type 2 diabetes and the metabolic syndrome is not yet fully understood. For this purpose the acquired data is put into a systems medicine environment to achieve a broader view on their functions. Furthermore I am trying to understand the chemical and biophysical mechanisms of oxidation in lipid droplets (C) which are the main constituent of adipocytes in adipose tissue. Non-enzymatic lipid peroxidation is based on an oxygen-centered radicals (e.g. hydroxyl radical) which cannot penetrate through the outer LDs phospholipid monolayer indicating that other mechanisms of radical reactions might govern generation of oxidized lipids in LDs, either involving longer lived, hydrophobic organic radicals or radical propagation from PL monolayer into the LD core.
mitarbeiter_mikelange_research
Education:
PhD Study (since 2016), Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, Germany
PhD thesis “Integration of oxidative stress into systems medicine view for obesity and obesity related complications”
Master of Science (2013-2015), Friedrich-Alexander Universität Erlangen-Nürnberg
Master thesis “Mechanistic Investigation of the oxPTM S-Sulfhydration”
Bachelor of Science (2010-2013), Friedrich-Alexander Universität Erlangen‑Nürnberg
Bachelor thesis “Synthesis of Triazole-Based PHD2 Inhibitors by Click-Chemistry”
Semester Abroad (2014), University College Dublin
Training and Studies in Biochemistry and Medicinal Chemistry
 
Prize/award:
  • 3rd Prize for the Best Young Investigator Contribution Poster on Lipidomics Forum 2017, 11, 2017, Borstel, Germany
 
Publications:
  • Zhixu Ni, Georgia Angelidou, Mike Lange, Ralf Hoffmann, and Maria Fedorova. "LipidHunter Identifies Phospholipids by High-Throughput Processing of LC-MS and Shotgun Lipidomics Datasets." Analytical Chemistry 89 (17), pp 8800–8807.
  • Cuevasanta, M. Lange, J. Bonanata, E. L. Coitino, G. Ferrer-Sueta, M. R. Filipovic, B. Alvarez, “Reaction of Hydrogen Sulfide with Disulfide and Sulfenic Acid to Form the Strongly Nucleophilic Persulfide”, J. Biol. Chem., 2015, 290(45) 26866-80.
  • Wedmann, C. Onderka, S. Wie, I. A. Szijarto, J. L. Miljkovic, A. Mitrovic, M. Lange, S. Savitsky, P. K. Yadav, R. Torregrossa, E. G. Harrer, T. Harrer, I. Ishii, M. Gollasch, M. E. Wood, E. Galardon, M. Xian, M. Whiteman, R. Banerjee, M. R. Filipovic, “Improved Tag-Switch Method Reveals that Thioredoxin Acts as Depersulfidase and Controls Intracellular Levels of Protein Persulfidation”, Chem. Sci., 2016, 7: 3414-26.